The HUNTER Trial Study Design

A Phase Ib/IIa, randomized, double-blind, placebo-controlled clinical trial evaluating the safety, tolerability, and preliminary efficacy of HSE-01 in adults with early type 2 diabetes.

Study Overview

The HUNTER Trial (HSE-T2D-001) represents the first-in-human evaluation of Hunteria umbellata seed extract (HSE-01) as an adjunct to intensive lifestyle intervention in adults with early type 2 diabetes. This Phase Ib/IIa study is designed to establish a preliminary safety and efficacy profile while generating critical dose-ranging information to inform larger Phase IIb remission trials.

Why This Study Matters

Early type 2 diabetes (diagnosed within 12–24 months) is potentially reversible. Landmark trials have demonstrated that up to 77% of patients can achieve remission with intensive lifestyle intervention yet no low-cost, orally available adjuvants exist to enhance these outcomes.

HSE-01 fills this gap. With a novel alpha-glucosidase inhibition mechanism distinct from metformin and a projected manufacturing cost of under $50/month, HSE-01 represents a scalable, accessible solution for global diabetes remission efforts.

This trial is fully aligned with FDA botanical drug development guidance (2016) and adheres to ICH-GCP E6(R2) standards. Upon completion, we will have the data foundation to pursue larger registration trials and potentially seek regulatory approval through the FDA botanical drug pathway.

Key Study Details

Official Title
HUNTER Trial: HSE-T2D-001

A Phase Ib/IIa Study of Hunteria umbellata Seed Extract for Early Type 2 Diabetes Remission

Trial Phase
Phase Ib/IIa

First-in-human safety, tolerability, and preliminary efficacy evaluation

Study Design
Randomized, Double-Blind, Placebo-Controlled

Three-arm parallel design with 1:1:1 randomization ratio

Sample Size
60 Participants

20 participants per arm (Low-dose HSE-01, High-dose HSE-01, Placebo)

Treatment Duration
12 Weeks + 2-Week Follow-Up

Total study participation: 14 weeks (screening to final safety check)

Primary Endpoints
Safety & Fasting Plasma Glucose

Adverse events, tolerability, change in FPG from baseline to Week 12

ClinicalTrials.gov
Registration Pending

Prospective registration before first participant enrollment

Estimated Completion
18 Months Post-Funding

From funding decision to final results publication

18-Month Project Timeline

Phase 1: Regulatory & Manufacturing

Months 1–3

IND application preparation and submission to FDA. GMP manufacturing of HSE-01 and matched placebo. Quality control testing (HPLC, microbial limits, stability studies). IRB approval. DSMB formation.

3 Months
Phase 2: Trial Setup

Months 2–4

Site staff training on protocol and GCP. Electronic data capture (eCRF) system configuration. Community outreach and recruitment planning. Participant screening materials finalized.

3 Months (overlapping)
Phase 3: Enrollment

Months 4–9

Screening 200 candidates to enroll 60 participants. Baseline assessments: labs, functional tests, dietitian counseling, exercise physiology sessions. Randomization and drug dispensing.

6 Months (10 participants/month)
Phase 4: Treatment & Monitoring

Months 5–15

12-week treatment period with clinic visits at Weeks 4, 8, and 12. Ongoing safety monitoring, home glucose tracking, adherence check-ins. Quarterly DSMB meetings. Week 14 post-treatment follow-up.

11 Months (rolling enrollment)
Phase 5: Data Analysis

Months 15–18

Database lock after last participant completes Week 14. Statistical analysis of primary and secondary endpoints. Safety report completion. Final DSMB review. Manuscript preparation.

4 Months
Milestone: Publication

Month 18+

Primary results manuscript submitted to high-impact journal (target: Diabetes Care, JCEM). Protocol paper published in Trials. ADA Scientific Sessions presentation. Phase IIb planning initiated if results positive.

Results Dissemination

Study Design Flow

1

Screening (≤2 Weeks Before Baseline)

Potential participants identified through community health centers, diabetes clinics, and online outreach. Eligibility confirmed via screening visit: HbA1c 6.5–9.0%, diagnosed ≤24 months, age 30–65, BMI 25–35. Informed consent obtained.

2

Baseline & Randomization (Week 0)

Comprehensive baseline assessment: fasting labs (glucose, HbA1c, insulin, C-peptide, lipids), meal tolerance test, body composition, functional capacity tests. Dietitian and exercise physiologist sessions. Stratified randomization (1:1:1) into three arms.

3

12-Week Treatment Period

Participants receive HSE-01 (low or high dose) or matching placebo once daily, plus intensive Mediterranean diet and structured exercise program (150 min/week). Clinic visits at Weeks 4, 8, and 12 for safety labs, adherence checks, and lifestyle counseling.

Arm 1

20

HSE-01 Low Dose + Lifestyle

Arm 2

20

HSE-01 High Dose + Lifestyle

Arm 3

20

Placebo + Lifestyle

4

Week 12 Final Assessment

Comprehensive endpoint evaluation: all baseline labs repeated, meal tolerance test, body composition, functional tests, quality of life questionnaires. Drug accountability and adverse event review.

5

Week 14 Safety Follow-Up

Phone call to assess post-treatment adverse events and well-being. Final data collection point before database lock. Participants transitioned to standard care with primary physician.

Study Endpoints

Primary Endpoints

  • Safety & Tolerability: Incidence and severity of adverse events, serious adverse events, and treatment discontinuations
  • Fasting Plasma Glucose (FPG): Change from baseline to Week 12 (primary efficacy endpoint)
  • Hypoglycemia Events: Frequency and severity (Level 1: 70-80 mg/dL; Level 2: <70 mg/dL; Level 3: <54 mg/dL or requiring assistance)
  • Laboratory Safety: Liver enzymes (ALT, AST), kidney function (creatinine, eGFR), complete blood count

Secondary Endpoints

  • HbA1c: Change from baseline to Week 12 (reflects 2-3 month glucose control)
  • Postprandial Glucose: 2-hour glucose during meal tolerance test
  • Insulin Sensitivity: HOMA-IR (homeostatic model assessment of insulin resistance)
  • Beta-Cell Function: Fasting C-peptide (marker of endogenous insulin secretion)
  • Lipid Profile: Total cholesterol, LDL, HDL, triglycerides
  • Body Composition: Weight, BMI, waist circumference
  • Blood Pressure: Systolic and diastolic measurements
  • Functional Capacity: 6-minute walk distance, 30-second chair stand test
  • Quality of Life: Diabetes Distress Scale (DDS-17)
  • Remission Rate: Proportion achieving HbA1c <6.5% (partial) or <5.7% (complete) without medication

Download Study Documents

Access our comprehensive protocol summary, investigator's brochure, and collaboration materials. Detailed documents are available to qualified partners upon request.